Flow Cytometry: Human TLR9 Stable Cell Line [NBP2-26272] - analysis of the TLR9 stable cell line. The assay was performed using the NF-kB SEAPorterâ„¢ Assay Kit. The Vector control stable cell line and TLR9 stable cell line were transfected with NF-kB/SEAP reporter plasmid for 16 h. Cells were stimulated with various amounts of CpG for 24 h followed by SEAP assay.
Flow Cytometry: Human TLR9 Stable Cell Line [NBP2-26272] - analysis of TLR9 expression in the TLR9 stable cell line. Intracellular expression of TLR9 in the TLR9 stable cell line was analyzed by flow cytometry using a PE-conjugated TLR9 antibody and compared with the Vector control stable cell line. Intracellular TLR Staining Flow Kit was used for this test.